Comparison of Erythrocyte Sedimentation Rate by Vision Principle and Westergren Method

Authors

  • Aayza Mujahid, Jawad Zaffar Choudry, Hamid Iqbal, Syeda Bint-E-Zahra, Saadia Latif, Zahra Habib

DOI:

https://doi.org/10.53350/pjmhs202317391

Abstract

Background: Erythrocyte sedimentation rate is a common test used as an assessment tool for inflammation since many years.

Aim: To compare erythrocyte sedimentation rate by automated ESR analyzer working on vision principle against conventional manual Westergren method.

Study design: Cross sectional study.

Methodology: A total number of 120 samples were taken from routine hospital patients whom ESR was requested through non-probability consecutive sampling following ethical approval. Samples were subjected to ESR estimation by manual Westergren and automated methods. Data was analyzed by SPSS version 22. Independent sample t-test used to compare erythrocyte sedimentation rate by automated ESR analyzer working on vision principle against conventional manual Westergren method with p-value ≤0.05 as significant. Pearson correlation was also applied.

Results: Mean ± SD for Manual Westergren method was 39.37±25.486 and Automated Vision principle was 25.83±21.015. Levene’s Test for Equality of Variances have p-values for ESR in patients with Manual Westergren method & Automated Vision principle was 0.018 with F-value 5.670. There was a non-linear relationship between the two methods (P value < 0.01).

Practical Implication: This study helped in exploring reliability of different ESR estimation methods as ESR results have notable impact on patient diagnosis and follow-up. With increasing demands and burden of patients in labs, new reliable and quick techniques for ESR determination are need of hour.

Conclusion: It was concluded that automated methods showed good  correlation  to manual Westergren  method thus are  reliable for ESR estimation. However, manual Westergren remained gold standard procedure for ESR estimation.

Keywords: Erythrocyte Sedimentation Rate, Reliability, Westergren Method and Automated Method.

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How to Cite

Aayza Mujahid, Jawad Zaffar Choudry, Hamid Iqbal, Syeda Bint-E-Zahra, Saadia Latif, Zahra Habib. (2023). Comparison of Erythrocyte Sedimentation Rate by Vision Principle and Westergren Method. Pakistan Journal of Medical & Health Sciences, 17(03), 91. https://doi.org/10.53350/pjmhs202317391